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dc.contributor.authorSadık, Demet Ataman
dc.contributor.authorBoyacı, İsmail Hakkı
dc.contributor.authorMutlu, Mehmet
dc.date.accessioned2020-09-17T14:43:37Z
dc.date.available2020-09-17T14:43:37Z
dc.date.issued30.11.2019
dc.identifier.citationSadık, D. A., Boyacı, İ. H., & Mutlu, M. (2019). Mixed monolayer decorated SPR sensing surface for thrombin detection. Journal of pharmaceutical and biomedical analysis, 176, 112822.en_US
dc.identifier.issn0731-7085
dc.identifier.otherarticle number 112822, number of pages 9
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2019.112822
dc.identifier.urihttp://hdl.handle.net/20.500.11851/3687
dc.description.abstractThe development of surface plasmon resonance (SPR) based immunosensor for thrombin detection was aimed. For this purpose, 3,3? Dithiodipropionic acid di (N-hydroxysuccinimide ester) (DSP):6-mercapto-1-hexanol (MCH) mixed self-assembled monolayers (mSAMs) were formed on gold surfaces for immobilization of anti-thrombin antibody. The performance of the immunosensor was determined against the target protein thrombin at various concentrations using flow cell coupled SPR. The linear detection range of the immunosensor was 30.0–100.0 nM with an R2 value of 0,992. Limit of Detection (LOD) and Limit of Quantification (LOQ) were determined to be 6.0 nM and 30.0 nM, respectively. The selectivity of the immunosensor was tested against a non-target model protein, human serum albumin (HSA) and the obtained ?RU value was found to be below the ?RU value corresponding to the LOQ concentration for thrombin. The immunosensor's capability to detect thrombin in diluted complex serum matrix was also tested and the obtained ?RU value (159 ± 16) was compared with ?RU value obtained for thrombin detection in PBS solution (137 ± 19). Based on the results, it was shown that DSP:MCH interface is a promising immobilization platform for binding biological recognition elements for the development of biosensors.en_US
dc.language.isoengen_US
dc.publisherElsevier B.V.en_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiosensoren_US
dc.subjectGold surfacesen_US
dc.subject3,3 ' Dithiodipropionic acid di (N-hydroxysuccinimide ester)en_US
dc.subjectDSP:6-mercapto-1-hexanol (MCH)en_US
dc.subjectMixed self-assembled monolayers (mSAMs)en_US
dc.subjectBiological recognition element immobilizationen_US
dc.subjectSurface plasmon resonance (SPR)en_US
dc.titleMixed monolayer decorated SPR sensing surface for thrombin detectionen_US
dc.typearticleen_US
dc.relation.journalJournal of Pharmaceutical and Biomedical Analysisen_US
dc.contributor.departmentTOBB ETU, Faculty of Engineering, Department of Biomedical Engineeringen_US
dc.contributor.departmentTOBB ETÜ, Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümütr_TR
dc.identifier.volume176
dc.relation.tubitakinfo:eu-repo/grantAgreement/TÜBİTAK/TBAG/211T103en_US
dc.contributor.orcidhttps://orcid.org/0000-0001-7146-1937
dc.identifier.wosWOS:000489000700021
dc.identifier.scopus2-s2.0-85071128263
dc.contributor.tobbetuauthorMutlu, Mehmet
dc.identifier.PubMedIDPMID:31454662
dc.identifier.doi10.1016/j.jpba.2019.112822
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.relation.otherHacettepe University, Scientific Research Projects Coordination Unit, Turkey [FHD-2016-8989]


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